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rosettesep human cd4+ t cell enrichment mixture no. 15062  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc rosettesep human cd4+ t cell enrichment mixture no. 15062
    Rosettesep Human Cd4+ T Cell Enrichment Mixture No. 15062, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosettesep+human+cd4+++t+cell+enrichment+mixture/rosettesep+human+b+cell+enrichment+cocktail/pm36197060-77-7-16
    Average 90 stars, based on 1 article reviews
    rosettesep human cd4+ t cell enrichment mixture no. 15062 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Inhibition of Effector Function but Not T Cell Activation and Increase in FoxP3 Expression in T Cells Differentiated in the Presence of PP14
    Article Snippet: The cells were cultured in RPMI medium (Biological Industries, Beit-Haemek, Israel) supplemented with 10% heat inactivated FCS, 2 mM glutamine and penicillin/streptomycin (Biological Industries) at 37°C, 5% CO2 incubator. .. CD4 + CD25 − naïve T cells were purified by first isolating CD4 + T cells from the venous blood of healthy donors using RosetteSep human CD4 T cell enrichment mixture (StemCell Technologies, Vancouver, Canada). .. Next, CD25 + cells were depleted using anti-CD25 microbeads with magnetic cell isolation system (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Isolation:

    Article Title: Superantigen enhancement of specific immunity: antibody production and signaling pathways.
    Article Snippet: PBMC were isolated by Histopaque (Sigma-Aldrich) density gradient centrifugation and viability was determined to be 95% by trypan blue exclusion. .. CD4 T cells were isolated from PBMC using the RosetteSep Human CD4 T cell enrichment mixture (StemCell Technologies, Vancouver, Canada) as per the manufacturer’s instructions. ..

    Article Title: Roles for CXC chemokine ligands 10 and 11 in recruiting CD4+ T cells to HIV-1-infected monocyte-derived macrophages, dendritic cells, and lymph nodes.
    Article Snippet: .. Primary CD4 T cells were isolated by negative selection from buffy coats using the RosetteSep human CD4 T cell enrichment mixture (StemCell Technologies). .. Isolated CD4 T cells were resuspended at 107 cells/ml in chemotaxis medium and preincubated for 2 h at 37°C with anti-CXCR3 (BD Pharmingen) or control IgG1 (R&D Systems) at 25 g/ml.

    Staining:

    Article Title: Mutations in early follicular lymphoma progenitors are associated with suppressed antigen presentation
    Article Snippet: Tumor B cells (CD5 − CD19 + CD10 + ) and tumor-infiltrating normal B cells (CD5 − CD19 + CD10 − ) were sorting using a FACS Aria II instrument (BD Biosciences). .. CD4 + T cells were obtained from peripheral blood of a single healthy male donor, using the RosetteSep Human CD4 T Cell Enrichment Mixture (Stemcell Technologies), and stained using the CellTrace Violet Cell Proliferation Kit (Life Technologies) according to the respective manufacturer’s instructions. ..

    Selection:

    Article Title: Roles for CXC chemokine ligands 10 and 11 in recruiting CD4+ T cells to HIV-1-infected monocyte-derived macrophages, dendritic cells, and lymph nodes.
    Article Snippet: .. Primary CD4 T cells were isolated by negative selection from buffy coats using the RosetteSep human CD4 T cell enrichment mixture (StemCell Technologies). .. Isolated CD4 T cells were resuspended at 107 cells/ml in chemotaxis medium and preincubated for 2 h at 37°C with anti-CXCR3 (BD Pharmingen) or control IgG1 (R&D Systems) at 25 g/ml.



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    Table S1.
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    Table S1.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: B vitamins attenuate the epigenetic effects of ambient fine particles in a pilot human intervention trial

    doi: 10.1073/pnas.1618545114

    Figure Lengend Snippet: Table S1.

    Article Snippet: We collected blood samples via venous phlebotomy (preexposure, immediately postexposure, and 24 h postexposure), and within 4 h, isolated CD4 + Th cells by removing unwanted cells using RosetteSep Human CD4 + T Cell Enrichment Mixture (no. 15062; Stem Cell Technologies).

    Techniques: Isolation